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PRACA PRZEGLĄDOWA
 
SŁOWA KLUCZOWE
DZIEDZINY
STRESZCZENIE
Wprowadzenie i cel:
Kleszcze to stawonogi odżywiające się krwią zwierząt i ludzi. Transmitują chorobotwórcze bakterie, wirusy i pierwotniaki, w tym krętkiBorrelia burgdorferi wywołujące boreliozę z Lyme i wirusa kleszczowego zapalenia mózgu. Hodowle komórkowe wyprowadzone z tkanek wek- torów znalazły szerokie zastosowanie w badaniach in vitro. Celem pracy był przegląd literatury dotyczącej kleszczowych linii komórkowych oraz ich zastosowania w badaniach naukowych.

Metody przeglądu:
W pracy opisano artykuły wyszukane w bazie PubMed na podstawie słów kluczowych: „kleszczowa linia komórkowa” (ang. tick cell line) lub „linia komórkowa z kleszczy” (ang. cell line from tick).

Opis stanu wiedzy:
Pierwszą kleszczową linię komórkową wyprowadzoną zRhipicephalus appendiculatus opisano w 1975 roku. Obecnie wyprowadzono ponad 50 ciągłych linii komórkowych z różnych gatunków kleszczy. Większość z nich należy do embrionalnych, czyli wyprowadzonych z jaj złożonych przez samicę kleszcza. Z gatunku Ixodes ricinus, najczęściej spotykanego w Polsce, dostępne są 4 linie komórkowe. Linie są szeroko wykorzystywane w badaniach naukowych nad biologią i fizjologią kleszczy, ich odpornością na akarycydy oraz poświęconych poznaniu układu immunologicznego kleszczy. Hodowle znalazły zastosowanie w badaniach interakcji wektor–patogen, charakterystyce mikroorganizmów oraz w izolacji licznych patogennych i niepatogennych drobnoustrojów ze środowiska.

Podsumowanie:
Skuteczność zastosowania kleszczowych linii komórkowych jako narzędzia badawczego potwierdza stale rosnąca liczba prac naukowych z ich wykorzystaniem. Ich użycie może mieć znaczenie dla opracowania skutecznej diagnostyki, profilaktyki oraz w leczeniu chorób odkleszczowych. Dzięki stale rozwijającym się nowym metodom badawczym kleszczowe linie komórkowe mogą być przydatnym narzędziem w przyszłych pracach naukowych.


Introduction and objective:
Ticks are blood-feeding parasites of animals and humans belonging to arthropods. They transmit pathogenic bacteria, viruses, and protozoa, including Borrelia burgdorferi (causative agent of Lyme borreliosis) and tick-borne encephalitis virus. Cell cultures derived from vector tissues are tools for a wide range of in vitro studies. The aim of the study was to review the literature on tick cell lines and their application in scientific research. Review methods. The terms “tick cell line” or “cell line from tick” were used in searching the PubMed® database

Review methods:
The terms “tick cell line” or “cell line from tick” were used in searching the PubMed® database.

Brief description of the state of knowledge:
The first tick cell line derived from Rhipicephalus appendiculatus was described in 1975. Currently, more than 50 continuous cell lines from various species have been derived. Most of them are embryonic and derived from eggs laid by female ticks. Four cell lines are available from the most common tick in Poland – Ixodes ricinus. The lines are widely used in research on the biology and physiology of ticks, their resistance to acaricides, and the understanding of the immune system of ticks. Cultures have been used in the study of vector-pathogen interactions, the characteristics of microorganisms, and for the isolation of from the environment.

Summary:
The effectiveness of tick cell lines as a research tool is confirmed by an increasing number of scientific reports. Their use may be important in the development of effective diagnosis, prevention, and treatment of tick-borne diseases. Tick cell lines may be a useful tool in future research through the development of new research methods.

Sawczyn-Domańska A, Zając V. Wykorzystanie kleszczowych linii komórkowych w badaniach patogenów odkleszczowych. Med Og Nauk Zdr. 2021; 27(4): 349–355. doi: 10.26444/monz/145008
 
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