Introduction and objective:
An excess of reactive oxygen species (ROS) generates oxidative stress, which can contribute to the development of lifestyle diseases. Health education is the most effective method of disease prevention, and knowledge about the body's defense mechanisms should be continuously expanded. The aim of this study was to assess the level of awareness regarding ROS and antioxidants among students of various fields at the University of Siedlce.

Material and methods:
The survey research was conducted among students of the Faculty of Science, Medical and Health Sciences, and Agricultural Sciences.

Results:
The best knowledge of ROS and antioxidant functions was demonstrated by Biology students, while the lowest was observed among students of Zoopsychology with Animal Therapy. The Internet was identified as the primary source of information about antioxidants, and awareness of the benefits of an antioxidant-rich diet was higher than knowledge about supplementation. Flavonoids and fat-soluble vitamins were the most commonly recognized antioxidants, while the role of vitamin C in collagen synthesis and the antioxidant effects of certain metals were less known.

Conclusions:
Most participants demonstrated a sufficient understanding of basic oxidative stress and antioxidant-related concepts, though the level of detailed knowledge varied by field of study. The results indicate insufficient awareness of certain important aspects of ROS, such as the impact of intense physical exercise on ROS production and their role in DNA damage. Students had a better understanding of the functions and dietary sources of natural antioxidants compared to the role of supplementation. Significant differences in knowledge among students from different field suggest the need to tailor educational programs to the specific needs of students.