RESEARCH PAPER
Effect of cycloporine A administration on aminothiols plasma concentration in children with idiopathic nephrotic syndrome
1
Klinika Pediatrii i Immunologii z Pododdziałem Nefrologii, Instytut Centrum Zdrowia Matki Polki w Łodzi,
2
Zakład Chemii Środowiska Uniwersytetu Łódzkiego
3
Klinika Hematologii, Onkologii i Diabetologii Dziecięcej, Uniwersytet Medyczny w Łodzi
Corresponding author
Marcin Tkaczyk
Klinika Pediatrii i Immunologii z Pod- Opinie o tym, czy ZN w dzieciństwie podobnie wpływa na oddziałem Nefrologii, Instytutu Centrum Zdrowia Matki Polki w Łodzi, ul Rzgowska 281/289, 93-338 Łódź
Klinika Pediatrii i Immunologii z Pod- Opinie o tym, czy ZN w dzieciństwie podobnie wpływa na oddziałem Nefrologii, Instytutu Centrum Zdrowia Matki Polki w Łodzi, ul Rzgowska 281/289, 93-338 Łódź
Med Og Nauk Zdr. 2013;19(1):59–63
KEYWORDS
ABSTRACT
Introduction:
Assessment of changes in plasma aminothiols metabolism in idiopathic nephrotic syndrome (INS) in children, with special consideration of the influence of cyclosporine A administration. Aminothiols are important elements of the red-ox balance in humans. The imbalance in red-ox status has been described in the nephrotic syndrome but may be a result of the disease itself or treatment (cyclosporine A).
Material and Methods:
The study group covered 95 nephrotic children divided into 3 groups, i.e. in acute phase of the disease (30), during steroid-induced (37), and steroid-free remission (28). In each group, children treated with Cyclosporine A were selected for the study, and a control group of 32 healthy children. Aminothiols in plasma were assessed by high-performance liquid chromatography. Protein bound and free fractions of aminothiols were measured.
Results:
Concentration of cysteine and homocysteine in nephrotic children were similar to controls. Only a few changes between stages of the disease were found. Free (toxic) homocysteine was elevated in early remission of INS. Glutathione concentration was not changed, whereas cysteinyloglycine concentration was decreased in first two stages of INS. Children treated with cyclosporine A showed significantly higher homocysteine and cysteine plasma concentrations in relapse and early remission of INS.
Conclusions:
Cyclosporine A may have a detrimental effect on the concentration of aminothiols promoting atherogenesis; however, the clinical importance of these observations requires further studies.
Assessment of changes in plasma aminothiols metabolism in idiopathic nephrotic syndrome (INS) in children, with special consideration of the influence of cyclosporine A administration. Aminothiols are important elements of the red-ox balance in humans. The imbalance in red-ox status has been described in the nephrotic syndrome but may be a result of the disease itself or treatment (cyclosporine A).
Material and Methods:
The study group covered 95 nephrotic children divided into 3 groups, i.e. in acute phase of the disease (30), during steroid-induced (37), and steroid-free remission (28). In each group, children treated with Cyclosporine A were selected for the study, and a control group of 32 healthy children. Aminothiols in plasma were assessed by high-performance liquid chromatography. Protein bound and free fractions of aminothiols were measured.
Results:
Concentration of cysteine and homocysteine in nephrotic children were similar to controls. Only a few changes between stages of the disease were found. Free (toxic) homocysteine was elevated in early remission of INS. Glutathione concentration was not changed, whereas cysteinyloglycine concentration was decreased in first two stages of INS. Children treated with cyclosporine A showed significantly higher homocysteine and cysteine plasma concentrations in relapse and early remission of INS.
Conclusions:
Cyclosporine A may have a detrimental effect on the concentration of aminothiols promoting atherogenesis; however, the clinical importance of these observations requires further studies.
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