PL EN
RESEARCH PAPER
The in vitro antimicrobial efficacy of Polish propolis and five plant extracts against selected bacteria and fungi
 
More details
Hide details
1
Medical University of Warsaw, Chair and Department of Medical Microbiology, Warsaw, Poland
 
 
Corresponding author
Marta Kierzkowska   

Warszawski Uniwersytet Medyczny, Polska
 
 
Med Og Nauk Zdr. 2019;25(2):107-111
 
KEYWORDS
TOPICS
ABSTRACT
Objectives:
Recently, there has been interest in the use of natural products or well-known propolys as antimicrobial agents. The aim of this study was to evaluate the antimicrobial properties of Polish propolis and Plantago lanceolate L., Quercus cortex, Uncaria tomentosa, Sideritis scardica, Trifolium pratense L. extracts, and the antimicrobial effects of propolis with several plant extracts available in local stores.

Material and methods:
The substances were tested for antimicrobial activity using disc diffusion test against 9 human pathogens: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus methicillin-resistant (MRSA), and sensitive to methicillin (MSSA), Staphylococcus epidermidis, Enterococcus faecalis, and clinical strains of Bacteroides fragilis, Candida albicans, Clostridium difficile.

Results:
Q. cortex extracts showed the strongest antimicrobial activity. The methanolic extract was stronger than the aqueous extract and exhibited significant antimicrobial activity against S. aureus, including MRSA strain, S. epidermidis and C. difficile. Polish propolys most efficiently inhibited the growth of S. aureus strains. All plants extracts and propolis were ineffective against B. fragilis. After simultaneous administration of propolys with aqueous and methanolic extracts of Q. cortex and Q. cortex with U. tomentosa, the antifungal effect was improved.

Conclusions:
1) Propolys possess better activity against Gram-positive than Gram-negative bacteria. 2) Q. cortex coadministered with propolys and Q. cortex together with U. tomentosa extract demonstrated stronger inhibitory effects against C. albicans than individual plant extracts.

 
REFERENCES (31)
1.
Nam SH, Choi YR, Jang SO, Shim YS, Han GS. Antimicrobial activity of propolys on different orał bacteria. Jndian J Sci Techno!. 2016; 9(15): 1-4. https://doi.org/l0.17485/ijst/... 15/89174.
 
2.
Karami L, Ghahtan N, Habibi H. Antibacterial Elfect of Plantago vvata and Lallemantia iberica Seed Extracts aga inst Some Bacteria. Res Mol Med (RMM). 2017; 5(3): 32-36. https://doi.org/l0.29252/rmm.5....
 
3.
Kuczer M, Czarniewska E, Majewska A, Różanowska M, Rosiński G, Lisowski M. Novel analogs of alloforon: Synthesis, conformational studies, pro-apoptotic and antiviral activity. Bioorg Chem. 2016; 66: 12-20. https:/ /doi.erg/ IO. I O 16/j.bioorg.2016 .03. 002.
 
4.
Martinotli S, Ranzato E. Propolys: a new fronlier for wound healingl Burns Trauma. 2015; 3: 9. https://doi.org/10.1186/s41038....
 
5.
Tosi B, Donini A, Romagnoli C, Brun A. Antimicrobial activity of some commercial exlracls of propolys prepared with different solvents, Phytother Res. 1996; IO: 335-336.
 
6.
Grecka K, Kuś PM. Okińczyc P, Worobo RW, Walkusz J. Szweda P. The Anti-Staphylococcal Potentia! of Ethanolic Polish Propolis Extracts. Molecules. 2019; 24(9): pii: E1732. https://doi.org/10.3390/ molecules24091732.
 
7.
Martinotti S, Pellavio G, Laforenza U, Ranzato E. Propolis lnduces AQPJ Expression: A Possible Way of Action in Wound Healing. Molcculea. 2019; 24(8): pii: El544. https://doi.org/l0.3390/molecu....
 
8.
Wolski T, Najda A, Kędzia B, Wolska-Gawron K. Phytotherapy of dermatological diseases [Polska]. Post Fitoter. 2017; 18: 298-308.
 
9.
Karima S, Sahli F, Zerroug MM. Antioxidanl and antimlcrobial activities of Plantago major. Int J Pharm Pharm Sc. 2015; 7(5): 58-64.
 
10.
Ferrazzano GF, Cant ile T, Roberto L, Ingenito A, Catania MR, Roscetto Ei wsp. Determination of the in vitro and in vivo antimicrobial activity on salivary Streptococci and Lactobacilli and chemical characterisation of the phenolic content of a Plant ago lanceolata infusion. Blomed Res lnt. 2015; 2015: 286817. http://dx.doi.org/l0.1155/2015....
 
11.
Pa rus A, Grys A. Plamago lanceolata L. - medicinal properties [Polska). Post Fitoter. 2010; 3: 162-165.
 
12.
AndrensekS, Simonovska B, Vovk I, Fyhrquist P, Vuorela H, Vuorela P. Antimicrobial and antioxidative enrichmentof oak(Quercus robur) bark by rotation planar extraction using ExtraChrom. lnt J Food Microbiol. 2004; 92(2): 181- 187. https://doi.org/10. IO 16/j .ijfoodmicro.2003.09.009.
 
13.
Wan NorAmilah WA, Masrah M, Hasmah A, Noor lzani NJ. In vitro antibacterial activity of Querc11s infectoria gali extrncts against multidrug resistant bacteria. Trop Biomed. 2014; 31(4): 680-688.
 
14.
Garcia R, Cayunao C, Bocie R, Backhouse N, Delporte C, Zaldivar M i wsp. Antimicrobial activity of isopteropodine. Z Naturforsch C. 2005; 60(5-6): 385-388.
 
15.
Ccahuana-Vasquez RA, Santos SS, Koga-I to CY, Jorge AO. Antimicrobial activityof Uncaria tome11tosa against orał human pathogens. Braz Orał Res. 2007; 21(1): 46-50.
 
16.
Kloucek P, Polesny Z, Svobodova B, Vlkova E, Kokoska L. Antibacterial screening of some Peruvian medicinal plants used in Calleria District. J Ethnopharmacol. 2005; 99(2): 309-312. https://doi.org/10.1016/j. jep.2005.01.062.
 
17.
Herrera DR, Tay LY, Rezende EC, Kozłowski VA Jr, Santos EB. In vitro antimicrobial activity of phytotherapic Uncaria tome11tosa against endodontic pathogens. J Orał Sci. 2010; 52(3): 473-476.
 
18.
Tadic V, Bojovic D, Arsie I, Dordević S, Aksentijevic K, Stamenić M i wsp. Chemical and antimicrobial evaluation of supercritical and conventional Sideritis scardica Griseb., Lamiaceae extracts. Molecules. 2012; 17(3): 2683-2703. https://doi.org/10.3390/molecu....
 
19.
Khan AV, Ahmed QU, Shukla I, Khan AA. Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pac f Trop Biomed. 2012; 2(3): 189-194. https://doi.org/10.1016/S222! -1691(12)60040-9.
 
20.
Wang SW, Chen Y, Joseph T, Hu M. Variable isoflavone content of red clover products affects intestinal disposition of biochanin A, formononetin, genistein, and daidzein. J Altem Complement Med. 2008; 14(3): 287-297. https://doi.org/l0.1089/acm.20....
 
21.
Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natura! products: a comprehensive review. Chin Med. 2018;13:20. https://doi.org/10.! 186/sl3020-0!8-0! 77-x –.
 
22.
Zabaiou N, Fouache A, Trousson A, Baron S, Zellagui A, Lahouel M i wsp. Biologica! properties of propolis extracts: Something new from an ancient product. Chem Phys Lipids. 2017; 207: 214-222. https://doi. org/10.! 016/j.chemphyslip.2017.04.005.
 
23.
Dziedzic A, Kubina R, Wojtyczka RD, Kabala-Dzik A, Tanasiewicz M, Morawiec T. The antibacterial effect of ethanol extract of polish propolis on mutans streptococci and lactobacilli isolated from saliva. Evid Bascd Complcment Alternat Med. 2013; 2013: 681891. http://dx.doi. org/10.1155/2013/681891.
 
24.
Bonamigo T, Campos JF, Alfredo TM, Balestieri JB, Cardoso CA, Paredes-Gamero EJ i wsp. Antioxidant, cytotoxic, and toxic activities of propolys from two native bees in Brazil: Scaptotrigona dep i/is and Melipona qundrifasciata antliidioides Oxid Med Cell Longev. 2017; 2017: 1038153. https://doi.org/10.1155/2017/1....
 
25.
das Neves MV, da Silva TM, Lima Ede O, da Cunha EV, Oliveira Ede J. Isoflavone formononetin from red propolys acts as a fungicide against Candida sp. Braz J Microbiol. 2016: 47(1):159-166. https://doi. . org/ I O. I O 16/j. bjm.2015.11. 009.
 
26.
Gucwa K, Kusznierewicz B, Milewski S, Van Dijck P, Szweda P. Antifungal Activity and Synergism with Azoles of Polish Propolis. 2018; 7(2):56. https://doi.org/l0.3390/pathog....
 
27.
Brown K, Valenta K, Fisman D, Simor A, Daneman N. Hospital ward antibiotic prescribing and the risks of Clostridium difficile infoction. JAMA Intern Med. 2015; 175(4): 626-633.
 
28.
Deryabin DG, Tolmacheva AA. Antibacterial and anti-quorum sensing molecular composition derived from Quercus cvrtex (Oak bark) Extract. Molecules. 2015; 20(9): 17093-17108. https://doi.org/10.3390/ molecules2009!7093.
 
29.
UAur A, Va rol O, Ceylan O. Antihacterial Activity of Sideritis curvidens. and Sideritis lematu. from Turkey. Pharmaceutical Biology. 2005; 43(1): 47-52. https:/ldoi.org/10.1080/13880200590903354.
 
30.
0zkan G, Sagdir; O, Ozcan M, Ozr;elik H, Onver A. Antioxidant and antlbacterial activities ofTurkish endemie Sideritis extructs. Grasas }' Aceiles. 2005; 56(1): 16-20.
 
31.
hltps:l/doi.org/10.3989/gya.2005.v56.il.129 31. Kargozar R, Azizi H, Salari R. A review of effective herba] mcdicincs in controlling menopausal symptoms. Electronic Physician. 2017; 9(1 1): 5826-5833. https:l/doi.org/10.19082/5826.
 
eISSN:2084-4905
ISSN:2083-4543
Journals System - logo
Scroll to top