Introduction and objective:
Protozoa of the genus Theileria transmitted by ticks cause a severe disease in animals, mainly ruminants. The aim of the project was to investigate the occurrence of protozoa Theileria spp. in Ixodes ricinus and Dermacentor reticulatus species using molecular biology methods in the Lublin Province.

Material and methods:
The research material was a group of 30 engorged female ticks removed from the host bodies. In laboratory conditions, the females laid eggs out of which some of the larvae emerged. Then, the isolated genetic material from females, eggs and larvae was analyzed by PCR using primers specific for the protozoa of the genus Theileria spp.

In the study group of 30 female ticks, 15 females of the species Ixodes ricinus and 15 females of the species Dermacentor reticulatus were identified. In total, females laid 6,720 eggs, of which 2,440 larvae hatched (36.31%). Both eggs and larvae were tested in pools of 20. Theileria protozoa were detected neither in the tested DNA material isolated from females nor in egg and larvae isolates.

In Poland, there are few studies on Theileria spp. The tests for Theileria spp. showed negative results in the genetic material of females, eggs and larvae in both species of ticks. This made it impossible to draw conclusions about the potential role of transovarial transmission as a pathway for the spread of Theileria spp. in the environment. Summing up, the obtained results did not confirm the participation of the common ticks in the transmission of Theileria spp.

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