Comparison of osteogenic differentiation ability between bone marrow-derived mesenchymal stem cells and adipose tissue-derived mesenchymal stem cells
More details
Hide details
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland
Paulina Kazimierczak   

Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland
Med Og Nauk Zdr. 2018;24(2):101–106
An important clinical problem is the fast restoration of large bone defects caused by trauma, tumour resection, infections, or skeletal anomaly. Autografts and allografts are commonly known approaches to bone repair, however, they have a lot of limitations. Bone tissue engineering has been considered as the alternative solution to bone rebuilding when natural grafts cannot be used. The primary model of bone tissue engineering comprises three elements: scaffold, growth factors, and stem or progenitor cells. The role of cells is to differentiate into osteoblasts and to form a bone extracellular matrix. Mesenchymal stem cells (MSCs) possess the mentioned features which make them a promising tool in supporting bone restoration process. MSCs are present in multiple tissues, including bone marrow and adipose tissue. This study presents the similarities and differences between bone marrow-derived mesenchymal stem cells (BMDSCs) and adipose tissue-derived mesenchymal stem cells (ADSCs). The study also compares the osteogenic potential of these cells, based on available literature. The presented comparison showed that both BMDSCs and ADSCs possess osteogenic ability under in vitro and in vivo conditions. However, most of the in vitro research confirmed the inferior osteogenic potential of ADSCs, compared to BMDSCs. Contrariwise, the in vivo studies revealed more controversies on this point in the scientific community; namely, some research studies considered the ADSCs as the promising alternative for BMDSCs which have been successfully used to-date for bone tissue engineering applications
1. Rose FR, Oreffo RO. Bone tissue engineering: hope vs hype. Biochem Biophys Res Commun. 2002;292(1):1-7.
2. Romagnoli C, Brandi ML. Adipose mesenchymal stem cells in the field of bone tissue engineering. World J Stem Cells. 2014;6(2):144-152.
3. Fröhlich M, Grayson WL, Wan LQ, Marolt D, Drobnic M, Vunjak-Novakovic G. Tissue engineered bone grafts: biological requirements, tissue culture and clinical relevance. Curr Stem Cell Res Ther. 2008;3(4):254-264.
4. Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg Am. 2004;86-A(7):1541-1558.
5. Kuhn LT, Liu Y, Boyd NL, Dennis JE, Jiang X, Xin X, et al. Developmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells. Tissue Eng Part A. 2014;20(1-2):365-377.
6. Daar AS, Bhatt A, Court E, Singer PA. Stem cell research and transplantation: science leading ethics. Transplant Proc. 2004;36(8):2504-2506.
7. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 1970;3(4):393-403.
8. Nöth U, Osyczka AM, Tuli R, Hickok NJ, Danielson KG, Tuan RS. Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells. J Orthop Res. 2002;20(5):1060-1069.
9. De Bari C, Dell'Accio F, Tylzanowski P, Luyten FP. Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum. 2001;44(8):1928-1942.
10. Bosch P, Musgrave DS, Lee JY, Cummins J, Shuler T, Ghivizzani TC, et al. Osteoprogenitor cells within skeletal muscle. J Orthop Res. 2000;18(6):933-944.
11. Nakahara H, Goldberg VM, Caplan AI. Culture-expanded human periosteal-derived cells exhibit osteochondral potential in vivo. J Orthop Res. 1991;9(4):465-476.
12. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317.
13. Lindroos B, Suuronen R, Miettinen S. The potential of adipose stem cells in regenerative medicine. Stem Cell Rev. 2011;7(2):269-291.
14. Meirelles Lda S, Nardi NB. Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br J Haematol. 2003;123(4):702-711.
15. Lennon DP, Caplan AI. Isolation of rat marrow-derived mesenchymal stem cells Exp Hematol. 2006;34(11):1606-1607.
16. Ringe J, Kaps C, Schmitt B, Büscher K, Bartel J, Smolian H, et al. Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages. Cell Tissue Res. 2002;307(3):321-327.
17. Takemitsu H, Zhao D, Yamamoto I, Harada Y, Michishita M, Arai T. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells. BMC Vet Res. 2012;8:150.
18. Eslaminejad MB, Nadri S. Murine mesenchymal stem cell isolated and expanded in low and high d6ensity culture system: surface antigen expression and osteogenic culture mineralization. In Vitro Cell Dev Biol Anim. 2009;45(8):451-459.
19. Soleimani M, Nadri S. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Nat Protoc. 2009;4(1):102-106.
20. Siclari VA, Zhu J, Akiyama K, Liu F, Zhang X, Chandra A, et al. Mesenchymal progenitors residing close to the bone surface are functionally distinct from those in the central bone marrow. Bone. 2013;53(2):575-586.
21. Akintoye SO, Lam T, Shi S, Brahim J, Collins MT, Robey PG. Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals. Bone. 2006;38(6):758-768.
22. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-147.
23. Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008;45(2):115-220.
24. Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, et al. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy. 2004;6(1):7-14.
25. Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, et al. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005;54(3):132-141.
26. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006;24(5):1294-1301.
27. Eslaminejad MB, Taghiyar L. Study of the structure of canine mesenchymal stem cell osteogenic culture. Anat Histol Embryol. 2010;39(5):446-455.
28. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211-228.
29. De Ugarte DA, Morizono K, Elbarbary A, Alfonso Z, Zuk PA, Zhu M, et al. Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs. 2003;174(3):101-9.
30. Przekora A, Vandrovcova M, Travnickova M, Pajorova J, Molitor M, Ginalska G, et al. Evaluation of the potential of chitosan/β-1,3-glucan/hydroxyapatite material as a scaffold for living bone graft production in vitro by comparison of ADSC and BMDSC behaviour on its surface. Biomed Mater. 2017;12(1):015030.
31. Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble JM, et al. Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem. 2006;99(5):1285-1297.
32. Im GI, Shin YW, Lee KB. Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Osteoarthritis Cartilage. 2005;13(10):845-853.
33. Liu TM, Martina M, Hutmacher DW, Hui JH, Lee EH, Lim B. Identification of common pathways mediating differentiation of bone marrow- and adipose tissue-derived human mesenchymal stem cells into three mesenchymal lineages. Stem Cells. 2007;25(3):750-760.
34. Shafiee A, Seyedjafari E, Soleimani M, Ahmadbeigi N, Dinarvand P, Ghaemi N. A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue. Biotechnol Lett. 2011;33(6):1257-1264.
35. Vishnubalaji R, Al-Nbaheen M, Kadalmani B, Aldahmash A, Ramesh T. Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis. Cell Tissue Res. 2012;347(2):419-427.
36. Hayashi O, Katsube Y, Hirose M, Ohgushi H, Ito H. Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue. Calcif Tissue Int. 2008;82(3):238-247.
37. Niemeyer P, Fechner K, Milz S, Richter W, Suedkamp NP, Mehlhorn AT, et al. Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma. Biomaterials. 2010;31(13):3572-3579.
38. Wen Y, Jiang B, Cui J, Li G, Yu M, Wang F, et al. Superior osteogenic capacity of different mesenchymal stem cells for bone tissue engineering. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013;116(5):324-332.
39. Kang BJ, Ryu HH, Park SS, Koyama Y, Kikuchi M, Woo HM, et al. Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton’s jelly for treating bone defects. J Vet Sci. 2012;13:299–310.
40. Stockmann P, Park J, von Wilmowsky C, Nkenke E, Felszeghy E, Dehner JF, et al. Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - a comparison of different tissue sources. J Craniomaxillofac Surg. 2012;40(4):310-320.
41. Brennan MA, Renaud A, Guilloton F, Mebarki M, Trichet V, Sensebé L, et al. Inferior In Vivo Osteogenesis and Superior Angiogeneis of Human Adipose Tissue: A Comparison with Bone Marrow-Derived Stromal Stem Cells Cultured in Xeno-Free Conditions. Stem Cells Transl Med. 2017;6(12):2160-2172.
42. Fennema EM, Tchang LAH, Yuan H, van Blitterswijk CA, Martin I, Scherberich A, et al. Ectopic bone formation by aggregated mesenchymal stem cells from bone marrow and adipose tissue: A comparative study. J Tissue Eng Regen Med. 2018;12:e150-e158.